Biological Activity:
1/4 dilution versus activated NHS assayed by immunodiffusion Not effective in Western Blots – SDS dissociates antigen. 1/500 to 1/2000 effective concentrations in ELISA
Item Number
R501371
Grouped product items
SKU
Size
Availability
Price
Qty
R501371-200μl
200μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
1/4 dilution versus activated NHS assayed by immunodiffusion Not effective in Western Blots – SDS dissociates antigen. 1/500 to 1/2000 effective concentrations in ELISA
Source
Rabbits (Oryctolagus cuniculus) immunized with highly purified human complement protein
Description
Extinction Coeff.
A280 nm = 1.35 at 1.0 mg/mL
Specificity
Monospecific for human SC5b-9 complex in plasma. No reaction occurs with non-activated normal human serum or plasma. Reacts with cell surface bound C5b-9 complex
General Description
Product is made from polyclonal antiserum from rabbits immunized with highly purified human complement protein. Antisera is stringently adsorbed with normal complement proteins to be monospecific for SC5b-9 neoantigen. Product sold is the purified IgG fraction. Rabbits are maintained in FDA certified facilities.
Physical Characteristics & Structure
Antibodies present in the antisera are primarily IgG.
Applications
Recognizes human SC5b-9 complex in complement-activated plasma.
Reacts with cell surface-bound C5b-9 complex after complement activation.
Western Blots: Not applicable. Antibody recognizes SC5b-9 neoantigen which isdestroyed upon denaturation in SDS.
ELISA: Effective at dilutions 1/500 to 1/2000 depending on conditions.
Immunodiffusion: Effective against purified SC5b-9 as well as SC5b-9 in complement-activated NHS or plasma at ¼ dilution.
