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SGVB⁺⁺ (with Ca⁺⁺ and Mg⁺⁺)

For research use only. We do not sell to patients.

Features and benefits
  • Specifications & Purity: 170 mM Sucrose, 0.1 % gelatin, 5 mM Veronal, 57 mM NaCl, 0.025 % NaN3 , 0.15 mM calcium chloride, and 0.5 mM magnesium chloride, pH 7.3.
Item Number
S501131
Grouped product items
SKU Size Availability Price Qty
S501131-250ml
250ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$396.90
S501131-1L
1L
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,063.90

Basic Description

Product Name SGVB⁺⁺ (with Ca⁺⁺ and Mg⁺⁺)
Specifications & Purity 170 mM Sucrose, 0.1 % gelatin, 5 mM Veronal, 57 mM NaCl, 0.025 % NaN3 , 0.15 mM calcium chloride, and 0.5 mM magnesium chloride, pH 7.3.
Description

General Description

SGVB⁺⁺ is a specialized buffer which is low in ionic strength (due to the low NaCl concentration), but isotonic (due to the sucrose). This combination of properties allows complement assays or binding assays to be performed with cells at low ionic strength, which improves binding, while the sucrose keeps the cells from lysing due to the high concentration of sucrose. This buffer is practically identical to DGVB buffer except that they use D-glucose instead of sucrose to maintain their isotonic characteristic. These buffers are isotonic with VBS, GVB, PBS, etc. meaning that the osmotic pressure on cell membranes will be the same even though the salt concentration is low. SGVB++ buffer is especially useful in C1 assays (Dodds, A.W. and Sim, R.B. (1997)) and in binding assays such as those measuring the binding of factor B or factor H to EsC3b cells (sheep erythrocytes bearing surface-bound C3b) (Pangburn, M.K.and Muller-Eberhard, H.J. (1978); Pangburn, M.K., et al. (1980)). These interactions are sensitive to thesalt concentration and lowering the ionic strength can enhance binding 5-to10-fold.


Buffer Components

Veronal is used as the buffer because in the mid-1900s this was the only buffer for pH range 7.2-7.4 that did not chelate metal ions and did not to inhibit complement reactions as did other buffers. Sodium chloride and sucrose are present to provide an isotonic environment so that cells do not lyse due to osmotic pressure. Gelatin is present to prevent loss of protein components due to adsorption onto tips or tubes during dilutions and in the assays themselves. 

Azide is present to prevent bacterial growth. Calcium is present because the classical and lectin pathways require it to hold the subunits of the C1, MBL and ficolin complexes together. Magnesium is required for formation of the C3 and C5 convertases of all three pathways of complement.


Although total calcium in plasma and serum is about 2.3 mM, the free available concentration is about 1.07 mM. The remainder is complexed with proteins or small molecules. Complement buffers contain 0.15 mM calcium because it was found that under CH50 assay conditions the classical pathway activity is greater at 0.15 mM than at 1.0 mM calcium. Plasma and serum concentrations of total magnesium are about 0.87 mM and the free available concentration is 0.5 mM. Thus, SGVB⁺⁺contains 0.5 mM magnesium chloride.


Physical Characteristics

The concentrationof gelatin in this buffer is below the concentration that forms solid gels at room temperature. However, at 4℃ some strings of gelatin can form during standing. They can be redissolved easily by heating to 37℃ or by brief heating in a microwave oven.


Applications

SGVB⁺⁺ can be used to titer both purified C1 complex and C1 in normal human serum samples (Dodds, A.W. and Sim, R.B. (1997)). In these assays all components are diluted in SGVB⁺⁺ and the EA cells are washed into this buffer. Binding assays such as those measuring the binding of C5, factor B or factor H to EsC3b cells (sheep erythrocytes bearing surface-bound C3b) or ZymosanC3b cells also benefit from being performed in SGVB++ (Pangburn, M.K. and Muller-Eberhard, H.J. (1978); Pangburn, M.K., et al. (1980)). The enhancement of binding can be as much as 10-fold compared to using a buffer with physiological ionic strength such as GVB or PBS.

Product Specifications

Form Liquid
Storage Temp Store at 2-8°C,Avoid repeated freezing and thawing
Shipped In Wet ice

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Associated Targets

CTH Tchem Cystathionine gamma-lyase 7 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

C7orf77 Tdark Uncharacterized protein C7orf77 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

C8orf34 Tbio Uncharacterized protein C8orf34 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

CGGBP1 Tbio CGG triplet repeat-binding protein 1 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

C8orf76 Tdark Uncharacterized protein C8orf76 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

CHD1 Tbio Chromodomain-helicase-DNA-binding protein 1 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

CHERP Tbio Calcium homeostasis endoplasmic reticulum protein 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

CHP1 Tbio Calcineurin B homologous protein 1 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

CAMTA2 Tbio Calmodulin-binding transcription activator 2 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

CNBD1 Tdark Cyclic nucleotide-binding domain-containing protein 1 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

CNBD2 Tdark Cyclic nucleotide-binding domain-containing protein 2 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

COMTD1 Tdark Catechol O-methyltransferase domain-containing protein 1 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

NCAPD3 Tbio Condensin-2 complex subunit D3 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

CMTR2 Tbio Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

CTNNBIP1 Tbio Beta-catenin-interacting protein 1 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

CNDP2 Tbio Cytosolic non-specific dipeptidase 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

PDE6H Tclin Retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

CTDNEP1 Tbio CTD nuclear envelope phosphatase 1 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

CNFN Tdark Cornifelin 0 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

CNKSR1 Tchem Connector enhancer of kinase suppressor of ras 1 30 Activities

Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)